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Improvement of the transient expression system for ...

Mar 19, 2018 · An efficient and high yielding expression system is required to produce recombinant proteins. Furthermore, the transient expression system can .

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The 32 kDa subunit of replication protein A (RPA ...

Dec 20, 2006 · The single-stranded cDNA was purified through quick Qiagen DNA purification column. This cDNA was then used for RACE–PCR. ... These results strongly suggested that both RPA32 and 70 might be involved in geminiviral DNA replication. This is the first report of the involvement of RPA32 and 70 kDa genes in the geminiviral DNA replication.

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(PDF) Geminivirus Isolation and DNA Extraction

Geminivirus Isolation and DNA Extraction. ... Ten geminiviral components and one alpha-satellite DNA were determined and compared to three geminiviral components obtained by conventional cloning ...

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Cloning, restriction mapping and phylogenetic ... - DeepDyve

Oct 01, 2006 · Read "Cloning, restriction mapping and phylogenetic relationship of genomic components of MYMIV from Lablab purpureus, Bioresource Technology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.

  • Published in: Bioresource Technology · 2006Authors: Sudheer Kumar Singh · Swastika Chakraborty · A K Singh · P K PandeyAbout: Begomovirus · Phylogenetic tree · Geminiviridae · Sequence analysis · DNA sequencing[PDF]Get Price

Extraction of geminiviral DNA from a highly mucilaginous ...

Plant Molecular Biology Reporter 18: 349-355, 2000 9 2000 International Society for Plant Molecular Biology.Printed in Canada. Protocols Extraction of Geminiviral DNA from a Highly Mucilaginous ...

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Extraction of High Quality DNA from Mucilaginous Plants ...

hindrance for the isolation of the high quality DNA and different downstream ofprocesses. Moreover, the chemotypic heterogeneity among species may cause interference in optimal DNA yields with a single DNA isolation protocol. Most of the DNA improvedextraction protocols need large amount of starting basedmaterial and extraction are

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A Facile & Cost-Effective DNA Isolation Method for ...

DNA isolation from Okra is quite difficult job due to polysaccharides and polyphenols. Even isolated DNA is not compatible with thermal reaction. A standard protocol of Wang et al. (1993) was reviewed and modified for DNA extraction from Okra leaf tissues within .

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The 32 kDa subunit of replication protein A (RPA ...

Dec 20, 2006 · The single-stranded cDNA was purified through quick Qiagen DNA purification column. This cDNA was then used for RACE–PCR. ... These results strongly suggested that both RPA32 and 70 might be involved in geminiviral DNA replication. This is the first report of the involvement of RPA32 and 70 kDa genes in the geminiviral DNA replication.

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MYMIV-AC2, a Geminiviral RNAi Suppressor Protein, Has ...

Genomic DNA Isolation and Genomic DNA PCR to Screen the MYMIV-AC2 and GFP Transgenics The genomic DNA was isolated from the plant tissue using the standard CTAB-based genome- DNA extraction protocol [26] and further used for molecular analysis.

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Optimization of a Viral System to Produce Vaccines

Optimization of a Viral System to Produce Vaccines and other Biopharmaceuticals in Plants by Andy Diamos A Dissertation Presented in Partial Fulfillment

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Multitasking in Replication Is Common among Geminiviruses

DNA extraction and purification. ... In conclusion, it still remains to be shown whether a geminiviral RDR mechanism completely relies on host factors or is promoted by a viral protein. ... Geminiviruses: models for plant DNA replication, transcription, and cell cycle regulation. Crit. Rev.

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A novel subviral agent associated with a geminivirus: The ...

2 prior to DNA extraction. Extraction and Analysis of DNA. Total nucleic acid was extracted as described previously (8) but included a treatment with RNase A. Inoculation sites were excised from the stem as described (16). DNA species were separated (5 mgylane) in 1.8% agarose gels containing 0.5mgzml21 ethidium bromide to

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DNA Extraction and Purification - Labome

DNA isolation methods are often modified and optimized for different cell types. Cetyltrimethylammonium bromide (CTAB) and guanidium thiocyanate (GITC) are often included in protocols for DNA extraction from plant materials, and are discussed more in detail in section "DNA extraction .

A novel role for RAD54: this host protein modulates ...

Geminiviruses primarily encode only few factors, such as replication initiator protein (Rep), and need various host cellular machineries for rolling-circle replication (RCR) and/or recombination-dependent replication (RDR). We have identified a host factor, RAD54, in a screen for Rep-interacting partners and observed its role in DNA replication of the geminivirus mungbean yellow mosaic India ...

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Functional Domains of a Geminivirus Replication Protein

The AL1 C terminus may also mediate interaction with other proteins, single-stranded DNA binding, nuclear localization, and/or attachment to the nuclear matrix. We are continuing to map the functional domains of TGMV AL1 to gain a more complete understanding of AL1 structure and function.

  • Published in: Journal of Biological Chemistry · 1997Authors: Beverly M Orozco · Ann B Miller · Sharon B Settlage · Linda HanleybowdoinAffiliation: North Carolina State UniversityGet Price

An improved method of DNA extraction from plants for ...

In this study, throughput (HTP) extraction (Bhattacharjee et al., 2009). For DNA extraction by HTP, leaves, stems, or mycelia were stored overnight a DNA extraction protocol was standardized, which is a at –80°C; seeds and tubers were treated at 65°C for 30 min before modification of the methods reported by Kang et al. being ground.

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AN EFFECTIVE METHOD FOR DNA EXTRACTION OF MATURE .

The existing extraction methods were not able to dissociate the metabolites contaminants of sapodilla ( Manilkara zapota (L.) van Royen) leaves and thus resulting in low quality of extracted DNA. The aim of this experiment was to develop an effective method to extract DNA from mature leaf samples of sapodilla ( Manilkara zapota (L.) van Royen).

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Regulation and functional analysis of a geminiviral DNA β

Regulation and functional analysis of a geminiviral DNA β satellite encoded gene by Omid Eini Gandomani M. Sc. (Hons) Plant Pathology, Tarbiat Modarres University, Tehran, Iran A thesis submitted for the degree of Doctor of Philosophy at The University of Adelaide, School of Agriculture, Food and Wine, Discipline of Plant and Food Science

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Rapid Purification and Size Separation of Gold ...

Purification and size-based separation of nanoparticles remain significant challenges in the preparation of well-defined materials for fundamental studies and applications. Diafiltration shows considerable potential for the efficient and convenient purification and size separation of water-soluble nanoparticles, allowing for the removal of small-molecule impurities and for the isolation of ...

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PPT – VIRAL DNA PURIFICATION PowerPoint presentation ...

GEMINIVIRAL DNA PURIFICATION. Geminiviruses are named by its shape under electron microscope; 11 GEMINIVIRAL DNA PURIFICATION. Four genus ; Mastrevirus ; Curtovirus ; Topocuvirus ; Begomovirus ; Begomovirus are whitefly transmitted; 12 GEMINIVIRAL DNA PURIFICATION. Begomovirus infect dicot plant species, such as tomato, potato, tobacco, pepper ...

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DNA Virus Vectors for Vaccine Production in Plants ...

Plants represent a safe, efficacious and inexpensive production platform by which to provide vaccines and other therapeutic proteins to the world's poor. Plant virus expression vector technology has rapidly become one of the most popular methods to express pharmaceutical proteins in plants. This review discusses several of the state-of-the-art plant expression systems based upon ...

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